Yeast Expression:
Please feel free to contact us for further information and to request a quote.
- Amplification/isolation of the gene of interest out of a customer-supplied vector.
- Cloning the gene of interest into a customer-supplied yeast expression vector.
- Verification of the authenticity of the subcloned gene by restriction enzyme digest and sequencing.
- Amplification and purification the recombinant vector DNA.
- Transformation of recombinant constructs into a customer-supplied yeast strain.
- Colony screening and Clone selection for the selection of a production strain.
- Creation of Cell Bank for the selected production strain.
- Test for the expression of the recombinant protein by ELISA or western blot (customer must provide appropriate antibodies) if desired.
- Expression and yield optimization method development available
- 1-10 liter of fermentation culture will be expressed and harvested for protein purification.
- Purification method development available
- Protein purification using classical chromatography media including: Ion exchange, hydrophobic interaction, and size exclusion chromatography.
- Protein purification using affinity tags at either native or denatured conditions.
Services offered: |
| I. Cloning of a gene of interest into an expression vector |
| II. Generation and identification of yeast colonies expressing target proteins |
| III. Large-Scale Culture |
| IV. Purification |
Contact us for further information and to request a quote.